Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in Clinical Specimens
نویسندگان
چکیده
منابع مشابه
application of multiplex pcr for detection and differentiation of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii.
background: entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica . multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. methods: for detection and differentiation of the three-microscopy indistinguishable entamoeba...
متن کاملApplication of Multiplex PCR for Detection and Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii
BACKGROUND Entamoeba moshkovskii and E. dispar are impossible to differentiate microscopically from the pathogenic species E. histolytica. Multiplex polymerase chain reaction (Multiplex PCR) is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. METHODS For detection and differentiation of the three-microscopy indistinguishable Entamoeba ...
متن کاملDifferentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii from diarrhoeic stools using Polymerase Chain Reaction in Kaduna, Nigeria
This is an Open Access article distributed under the terms of the creative commons Attribution 4.0 licence (http://creativecommons.org/licenses/by/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii from diarrhoeic stools using Polymer...
متن کاملPCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.
This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is t...
متن کاملReal-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples.
A closed-tube, real-time PCR assay was developed for sensitive and specific detection and differentiation of the two closely related intestinal protozoan parasites Entamoeba histolytica and Entamoeba dispar directly from human feces. The assay is performed with the LightCycler system using fluorescence-labeled detection probes and primers amplifying a 310-bp fragment from the high-copy-number, ...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2010
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.2010.10-0050